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Antarctic cryosphere fungal diversity (2015)
Citatie
de Menezes G, Camara P, Pinto O, Convey P, Calvarho-Silva M, Simões J, Rosa C, Rosa L (2021): Antarctic cryosphere fungal diversity (2015). v1.8. SCAR - Microbial Antarctic Resource System. Dataset/Metadata. https://ipt.biodiversity.aq/resource?r=cryosphere_fungi_antarctica_2015&v=1.8 https://doi.org/10.15468/wwbr57
Contact:
Rosa, Luiz Henrique Beschikbaarheid:
![]() Nota: The publisher and rights holder of this work is SCAR - Microbial Antarctic Resource System. This work is licensed under a Creative Commons Attribution (CC-BY) 4.0 License.
Beschrijving
Amplicon sequencing dataset (Illumina MiSeq) targeting Fungi (ITS) in ice samples (n=8) from the Antarctic Peninsula. meer
Samples were collected using sterile suits and gloves. Each sample was broken into smaller pieces, and surface decontamination carried out using 5% sodium hypochlorite (10 s), sterilized distilled water (10 s), and exposure to ultraviolet radiation (10 min). Glacial ice fragments of approximately 20 kg mass, were collected adjacent to the ice fronts of seven marine terminating glaciers in the South Shetland Islands and the north-west Antarctica Peninsula during the austral summer season in December 2015 and December 2016. The samples were melted and a total of 12–15 L of the resulting water Filtered through 47 mm diameter (Millipore) membranes (three membranes per sampling site, each using 4–5 L) until each membrane became saturated. Membranes were then stored at -20°C until DNA extraction. The three membranes resulting from filtering the melted ice from each sampling site were processed together in order to increase DNA yield. Total DNA was extracted using 0.5 mL extraction buffer [sodium dodecyl sulfate (SDS) 10%], left at 55°C for 18 h, followed by 165 μL NaCl (5 M) and 165 μL cetyltrimethylammonium bromide (CTAB, 10%), then 600 μL chloroform was added and the mixture centrifuged (Eppendorf/Germany) at 13,000 rpm for 10 min. The supernatant was cleaned using the QIAGEN Dneasy PowerClean cleanup Kit. The ITS2 region was used as a DNA barcode, using the universal primers ITS3 and ITS4 and were sequenced at Macrogen Inc. (South Korea) on an Illumina MiSeq sequencer, using the MiSeq Reagent Kit v3 (600-cycle) following the manufacturer’s protocol. Scope Thema's: Biologie > Ecologie - biodiversiteit Kernwoorden: Zoet water, Terrestrisch, Glaciers, Metadata, Antarctica, Antarctic Peninsula, Fungi Geografische spreiding Spreiding in de tijd
1 December 2015 - 31 December 2016 Taxonomic coverage
Fungi [WoRMS]
Parameter
DNA Bijdrage door
Gerelateerde datasets
Dataset status: Afgelopen
Data type: Meta database
Data oorsprong: Onderzoek: veldexperiment
Metadatarecord aangemaakt: 2021-07-05
Informatie laatst gewijzigd: 2021-07-05
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