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Evolutionary affiliation of the marine nitrogen-fixing cyanobacterium Trichodesmium sp. strain NIBB 1067, derived by 16S ribosomal RNA sequence analysis
Wilmotte, A.; Neefs, J.-M.; De Wachter, R. (1994). Evolutionary affiliation of the marine nitrogen-fixing cyanobacterium Trichodesmium sp. strain NIBB 1067, derived by 16S ribosomal RNA sequence analysis. Microbiology 140(8): 2159-2164. https://hdl.handle.net/10.1099/13500872-140-8-2159
In: Microbiology. Society for General Microbiology: Reading. ISSN 1350-0872; e-ISSN 1465-2080, more
Peer reviewed article  
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Keywords
    Cyanobacteria [WoRMS]; Trichodesmium Ehrenberg ex Gomont, 1892 [WoRMS]; Trichodesmium Ehrenberg ex Gomont, 1892 [WoRMS]
    Marine/Coastal
Author keywords
    Trichodesmium , 16S rRNA sequence analysis, taxonomy, cyanobacteria, internal transcribed spacer
Authors  Top 
  • Wilmotte, A., more
  • Neefs, J.-M.
  • De Wachter, R.
Abstract
    The 16S rRNA sequence of sp. strain NIBB 1067 was determined and used for the construction of a distance tree and bootstrap analysis. The tree shows that, among the available cyanobacterial 16S rRNA sequences, NIBB 1067 has PCC 7515 as its closest relative, presenting 94.9% of sequence similarity with the latter strain. This is in contrast to a difference of 9 mol% G + C in mean genomic DNA base composition between the two organisms. Nevertheless, the genotypic heterogeneity presented by a number of strains assigned to the genus hinders a taxonomic decision on the separate existence of the genera and The sequence of the internal transcribed spacer (ITS) between the 16S and 23S rRNA genes was also determined, as a possible marker to study inter- and intraspecific variability. The ITS contains the genes coding for tRNAlle and tRNAAla and its total length is 547 nucleotides. In six out of eight sequenced clones, there is a duplication of 29 nucleotides, surrounding the 5′ end of the tRNAlle.
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