Does ammonia trigger hyperventilation in the elasmobranch, Squalus acanthias suckleyi?
De Boeck, G.; Wood, C.M. (2015). Does ammonia trigger hyperventilation in the elasmobranch, Squalus acanthias suckleyi? Resp. Physiol. Neurobiol. 206: 25-35. dx.doi.org/10.1016/j.resp.2014.11.009
We examined the ventilatory response of the spiny dogfish, to elevated internal or environmental ammonia. Sharks were injected via arterial catheters with ammonia solutions or their Na salt equivalents sufficient to increase plasma total ammonia concentration [TAram la by 3-5 fold from 145 21 p,M to 447 150 p,M using NH4HCO3 and a maximum of 766 1001.LM using (NH4)2SO4. (NH4)2SO4 caused a small increase in ventilation frequency (+14%) and a large increase in amplitude (+69%), while Na2SO4 did not. However, CO2 partial pressure (Paco2) also increased and arterial pHa and plasma bicarbonate concentration ([HCO3-1a) decreased. NH4HCO3 caused a smaller increase in plasma ammonia resulting in a smaller but significant, short lived increases in ventilation frequency (+6%) and amplitude (36%), together with a rise in Paco2 and [HCO31a. Injection with NaHCO3 which increased pHa and [HCO3la did not change ventilation. Plasma ammonia concentration correlated significantly with ventilation amplitude, while ventilation frequency showed a (negative) correlation with pHa. Exposure to high environmental ammonia (1500 p,M NH4HCO3) did not induce changes in ventilation until plasma [TAran]a increased and ventilation amplitude (but not frequency) increased in parallel. We conclude that internal ammonia stimulates ventilation in spiny dogfish, especially amplitude or stroke volume, while environmental ammonia only stimulates ventilation after ammonia diffuses into the bloodstream. 0 2014 Elsevier B.V. All rights reserved.
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