Effects of shrimp-fish polyculture on immune parameters, disease resistance of white shrimp and the prevalence of Vibrio spp
Hoang, M.N.; Nguyen, P.N.; Bossier, A.M.V.E.M.; Bossier, P. (2022). Effects of shrimp-fish polyculture on immune parameters, disease resistance of white shrimp and the prevalence of Vibrio spp. Aquac. Res. 53(4): 1316-1326. https://dx.doi.org/10.1111/are.15666
In: Aquaculture Research. Blackwell: Oxford. ISSN 1355-557X; e-ISSN 1365-2109, more
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| Keywords |
Penaeus vannamei Boone, 1931 [WoRMS]; Vibrio Pacini, 1854 [WoRMS]; Vibrio alginolyticus (Miyamoto, Nakamura & Takizawa, 1961) Sakazaki, 1968 [WoRMS]
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| Author keywords |
immune response; monoculture; shrimp polyculture; Vibrio |
| Authors | | Top |
- Hoang, M.N., more
- Nguyen, P.N.
- Bossier, A.M.V.E.M., more
- Bossier, P., more
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| Abstract |
We pre-grew shrimp under polyculture (shrimp-tilapia ‘ST’ and shrimp-mullet ‘SM’) and monoculture (P-control) conditions until they reached the size of 10–12 g. Then, the shrimps were collected and injected with Vibrio alginolyticus (2.1 × 105 CFU shrimp−1). Some shrimp, originating from the shrimp monoculture, was also injected with sterile saline solution, served as N-Control. Over 8 days, mortality in N-control was significantly lower than that in the other treatments (p = 0.001). Total haemocyte counts (THC), phenoloxidase activity (PO) and respiratory burst activity (RB) in haemocytes decreased sharply after injection and were significantly different in P-control, ST and SM as compared to N-control (p < 0.05). However, there were no significant differences in shrimp survival and immune parameters among P-control, ST and SM (p > 0.05). It is concluded that the application of shrimp-fish polyculture systems does not contribute to the robustness of cultured shrimp as verified by challenge through injection. The second experiment was carried out to verify the effect of shrimp-mullet polyculture on the prevalence of Vibrio spp. Mullet (3.45 g) were supplemented (either biomass of 500 or 700 g m−3) into six aquaria where shrimp (0.48 g) was stocked at a density of 80 g m−3. The other six aquaria were just stocked with shrimp as P-control and N-control. Aquaria were inoculated with V. harveyi at a density of 103 CFU ml−1, except for N-control. Results showed that the presence of mullet at either biomass of 500 or 700 g m−3 could reduce TCBS counts in water by 20.9% and 24.3%, respectively, as compared to shrimp monoculture. |
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