Determination and localization of oil components in living benthic organisms by fluorescence microscopy
Zeeck, E. (1980). Determination and localization of oil components in living benthic organisms by fluorescence microscopy. Helgol. Meeresunters. 33(1-4): 272-277. https://dx.doi.org/10.1007/BF02414752
In: Helgoländer Meeresuntersuchungen. Biologische Anstalt Helgoland: Hamburg. ISSN 0174-3597, meer
Ook verschenen in:
Kinne, O.; Bulnheim, H.-P. (Ed.) (1980). Protection of life in the sea: 14th European Marine Biology Symposium, 23-29 September 1979, Helgoland. European Marine Biology Symposia, 14. Helgoländer Meeresuntersuchungen, 33(1-4). 772 pp., meer
Microspectrofluorometric measurements are made to determine uptake and distribution of oil in marine organisms after exposure to crude oil. Equipment combining fluorescence microscopy with spectral analysis of the fluorescence emission is described. After contamination with oil, the intestine content of Lumbricillus lineatus, Nereis diversicolor and Anaitides mucosa shows a fluorescence emission at long wavelengths with a maximum at about 550 nm; this is in contrast to the fluorescence emission of these organisms without oil contamination. There is evidence that aromatic hydrocarbons are metabolized in the intestine of the worms studied.
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