Pluripotency and chimera competence of an embryonic stem cell line from the sea perch (Lateolabrax japonicus)
Chen, S.-L.; Sha, Z.X.; Ye, H.-Q.; Liu, Y.; Tian, Y.-S.; Hong, Y.; Tang, Q.-S. (2007). Pluripotency and chimera competence of an embryonic stem cell line from the sea perch (Lateolabrax japonicus). Mar. Biotechnol. 9(1): 82-91. http://dx.doi.org/10.1007/s10126-006-6050-1
In: Marine Biotechnology. Springer-Verlag: New York. ISSN 1436-2228; e-ISSN 1436-2236, meer
A stable GFP-expressing (GFP+LJES1) cell strain was developed from the LJES1 cells obtained from sea perch (Lateolabrax japonicus,) embryos. GFP+LJES1 cells were induced in vitro by RA to differentiate into a variety of cell types and also had the ability to form embryoid body-like structures in suspension culture. To determine the differentiation potential of LJES1 cells in vivo, GFP+LJES1 cells were transplanted into sea perch and zebrafish embryos at mid-blastula stage. Twenty out of 478 transplanted sea perch embryos contained GFP-expressing LJES1 cells 24 h after microinjection. Fifteen chimera embryos developed into fry. In these chimeras, the GFP+LJES1 cells contributed to a variety of tissues including the head and trunk. In zebrafish, 221 embryos were microinjected with GFP+LJES1 cells and 22 chimera embryos and fries expressing GFP were obtained. Donor GFP+LJES1 cells contributed to various tissues in head and trunk of zebrafish embryos and hatched fry.
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