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Proximal to distal gradient of luminescence in the arm of Amphiura filiformis (Echinodermata-Ophiuroidea)
Delval, S.; Mallefet, J. (2010). Proximal to distal gradient of luminescence in the arm of Amphiura filiformis (Echinodermata-Ophiuroidea), in: Harris, L.G. et al. Echinoderms: Durham - Proceedings of the 12th international echinoderm conference, Durham, New Hampshire, USA, 7-11 August, 2006. pp. 355-357
In: Harris, L.G. et al. (2010). Echinoderms: Durham - Proceedings of the 12th international echinoderm conference, Durham, New Hampshire, USA, 7-11 August, 2006. CRC Press: [s.l.]. ISBN 978-0-415-40819-6. I–XXXVI pp. http://dx.doi.org/10.1201/9780203869543-f, meer

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Documenttype: Congresbijdrage

Trefwoord
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Abstract
    Bioluminescence is a widespread phenomenon. Among benthic phyla, echinoderms contain some luminous representatives. The emission of visible light originates from a biochemical reaction where chemical energy is transformed into luminous energy. Two bioluminescent systems exist to date: the luciferin-luciferase and the photoprotein one. We here report for the first time the presence of a luciferin-luciferase system in Amphiura filiformis. New assay methods have been developed to detect luciferin and luciferase contents in A. filiformis tissues. This work shows that luciferin/luciferase contents increase from arm base to the arm tip; this increase parallels the observed raise of the light intensity induced by potassium chloride (KCl) depolarisation of isolated arm. This new approach will provide a useful tool to study functional recovery of luminous capabilities during regeneration in Amphiura filiformis.

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