Isolation and multiplex analysis of six polymorphic microsatellites in the Antarctic notothenioid fish, Trematomus newnesi
Van Houdt, J.K.J.; Hellemans, B.; Van de Putte, A.; Koubbi, P.; Volckaert, F.A.M. (2006). Isolation and multiplex analysis of six polymorphic microsatellites in the Antarctic notothenioid fish, Trematomus newnesi. Mol. Ecol. Notes 6(1): 157-159. dx.doi.org/10.1111/j.1471-8286.2005.01174.x
Six polymorphic microsatellites were isolated from the dusky notothen, Trematomus newnesi (Boulenger 1902), using a microsatellite enrichment protocol and selective hybridization with di- tetra- and penta-repeat probes. The loci were screened in 48 individuals captured in the Southern Ocean (coastal zone of Terre-Adelie), revealing eight to 22 alleles per locus and an observed heterozygosity ranging from 0.70 to 0.92. These microsatellite markers provide a tool to study the relationship between the various morphs observed in this species and can be used for population genetic analysis and biodiversity studies.
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